ABSTRACT
Informe que presenta los resultados de las encuestas realizadas según las recomendaciones de la OMS, para la vigilancia de resistencia pretratamiento y resistencia adquirida del VIH.
Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Drug Resistance/drug effects , HIV Infections/drug therapy , HIV-1/drug effects , Anti-Retroviral Agents/therapeutic use , Suppression , Epidemiologic Methods , Anti-Retroviral Agents/blood , GuatemalaABSTRACT
The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using a single-step deproteinization. Chromatographic separation of zidovudine from interfering components was achieved with a C-18 reverse phase column, a mobile phase consisting of a mixture of sodium acetate buffer (55mM) with pH adjusted to 7.0 and acetonitrile (91:9 v/v) and UV detection set at 265 nm. The method was linear from 100 to 10000 ng.mL"¹ (r² > 0.9995), and zidovudine had a mean recovery from plasma of 92.8 percent. The coefficient of variation of inter-day and intra-day quality control samples was less than 15 percent. After a single intranasal dose of zidovudine administered to rats, pharmacokinetic parameters (AUC0 24, Cmax, t , t1/2) were determined. The proposed method was found to be simple, specific, accurate, and precise and could be applied to the quantitative analysis of clinical pharmacokinetic studies of zidovudine in rats.